Scientific News | Research: Using a new cell delivery method to help cells adhere

Washington [US], Jun 7 (ANI): According to the results of research conducted by the College of Engineering at Carnegie Mellon University, cell therapies have long been considered an alternative treatment option for patients with various diseases caused by organs and tissues. heart failure, including heart attack, diabetes, corneal blindness and cystic fibrosis. Although excellent in theory, in practice these therapies have limited clinical success in many applications due to low cell viability after injection, as well as poor retention at the injection site and graft. in damaged tissue.

Ongoing research by BME’s Rachelle Palchesko and Adam Feinberg explores the use of a new method of cell delivery to help cells stay where they’re needed most.

Read also | The lineup for 2022 Made in America has been revealed.

Bad Bunny and Tyler, the creator… – Pop Crave’s latest tweet.

More than 50,000 corneal transplant procedures are performed in the United States each year, an impressive statistic that exceeds the number of transplants of all other solid organs combined. In new research published in Communications Materials, researchers from CMU and the University of Pittsburgh propose using a small shrink-wrapped packet of corneal endothelial cells as a potential alternative to corneal transplantation when a low cell density endothelial is the cause of corneal blindness.

The corneal endothelium (CE) is a single layer of cells that lines the back surface of the cornea and is responsible for maintaining the thickness and clarity of the cornea. Nearly half of all corneal transplants come from EC failure, primarily due to loss of cells that cannot replicate to repair damage or injury.

Read also | Italy vs Hungary Live Online UEFA Nations League 2022-23: Get Free Broadcast Time on IST and TV Channels to Watch ITA vs HUN Football Match in India.

Although some treatments for EC failure exist, chronic rejection and the limited supply of donors have motivated the development of new methods to inject EC cells to repopulate the corneal endothelium and restore function. Until now, most approaches required the existing CE to be removed by scraping or cryo-injuring the cornea to provide a place for the delivered cells to attach.

“You can imagine that if you try to take a healthy cell and put it in a hostile tissue, it doesn’t want to stay there,” explained Rachelle Palchesko, a researcher at the special faculty of biomedical engineering. “We had a benchmark for the effective application of shrink-wrapped cells in the cornea based on work done by a group in Japan, and we knew we could improve on it. We were able to show that we can wrap cells efficiently and integrate them into tissues at high density, without inducing injury or eliminating cells.Our technology can improve cell therapies and help cells stay where we want them.

The group’s technique uses retractable micro-patterned islands of corneal endothelial cells in a basement membrane-like extracellular matrix layer that allows cells to maintain their cell-cell junctions and cytoskeletal structure in suspension. In a series of studies, the small cell bundles showed an ability to rapidly engraft into high-density intact corneal endothelial monolayers in in vitro and in vivo model systems.

“Most of my research has focused on the treatment of corneal blindness, but we believe this technology has great potential to be applied to other areas of the body,” Palchesko said. “Our lab group is studying how to apply this technology to treat cystic fibrosis or deliver cells after a heart attack.”

“Imagine if organ failure could be prevented with a simple injection into the affected tissue instead of waiting for a transplant that might never come,” said Adam Feinberg, professor of biomedical engineering and materials science and engineering. . “This is the truly exciting potential of the technology as it is developed and validated. And we are grateful for the support of the National Institutes of Health and the Cystic Fibrosis Foundation in funding this research.”

Palchesko added, “It’s a simple and effective technology — it’s not overly designed; we just pack these cells in small packages. I think we can go further and help a lot of people. Cell therapies have long been considered an alternative treatment option for patients with various diseases caused by organ and tissue failure, including heart attack, diabetes, corneal blindness and cystic fibrosis. Although excellent in theory, in practice these therapies have limited clinical success in many applications due to low cell viability after injection, as well as poor retention at the injection site and graft. in damaged tissue. Ongoing research by BME’s Rachelle Palchesko and Adam Feinberg explores the use of a new method of cell delivery to help cells stay where they’re needed most.

More than 50,000 corneal transplant procedures are performed in the United States each year, an impressive statistic that exceeds the number of transplants of all other solid organs combined. In new research published in Communications Materials, researchers from CMU and the University of Pittsburgh propose using a small shrink-wrapped packet of corneal endothelial cells as a potential alternative to corneal transplantation when a low cell density endothelial is the cause of corneal blindness.

The corneal endothelium (CE) is a single layer of cells that lines the back surface of the cornea and is responsible for maintaining the thickness and clarity of the cornea. Nearly half of all corneal transplants come from EC failure, primarily due to loss of cells that cannot replicate to repair damage or injury.

Although some treatments for EC failure exist, chronic rejection and the limited supply of donors have motivated the development of new methods to inject EC cells to repopulate the corneal endothelium and restore function. Until now, most approaches required the existing CE to be removed by scraping or cryo-injuring the cornea to provide a place for the delivered cells to attach.

“You can imagine that if you try to take a healthy cell and put it in a hostile tissue, it doesn’t want to stay there,” explained Rachelle Palchesko, a researcher at the special faculty of biomedical engineering. “We had a benchmark for the effective application of shrink-wrapped cells in the cornea based on work done by a group in Japan, and we knew we could improve on it. We were able to show that we can wrap cells efficiently and integrate them into tissues at high density, without inducing injury or eliminating cells.Our technology can improve cell therapies and help cells stay where we want them.

The group’s technique uses retractable micro-patterned islands of corneal endothelial cells in a basement membrane-like extracellular matrix layer that allows cells to maintain their cell-cell junctions and cytoskeletal structure in suspension. In a series of studies, the small cell bundles showed an ability to rapidly engraft into high-density intact corneal endothelial monolayers in in vitro and in vivo model systems.

“Most of my research has focused on the treatment of corneal blindness, but we believe this technology has great potential to be applied to other areas of the body,” Palchesko said. “Our lab group is studying how to apply this technology to treat cystic fibrosis or deliver cells after a heart attack.”

“Imagine if organ failure could be prevented with a simple injection into the affected tissue instead of waiting for a transplant that might never come,” said Adam Feinberg, professor of biomedical engineering and materials science and engineering. . “This is the truly exciting potential of the technology as it is developed and validated. And we are grateful for the support of the National Institutes of Health and the Cystic Fibrosis Foundation in funding this research.”

Palchesko added, “It’s a simple and effective technology — it’s not overly designed; we just pack these cells in small bundles. I think we can go further and help a lot of people. (ANI)

(This is an unedited and auto-generated story from syndicated newsfeed, LatestLY staff may not have edited or edited the body of the content)

Source link

About Donald P. Hooten

Check Also

Is it a lie? New scientific method to detect the truth

UN Security Council faces new draconian demands for reform after failing to prevent Russian invasion …